Study on The Relationship Between Glucoeoaicoid Receptors and Curative Effect of Sudden Sensorineural Hearing Loss

LIU Qianxu; ZHAO Xiaoming; WANG Zhiwei; LUO Jialong

doi:10.3969/j.issn.1672-2922.2015.02.036

Abstract：Objective To investigate the relationship between expression of glucocorticoid receptor(GR) in peripheral blood mononuclear cells(PBMCs)of patients with sudden sensorineural hearing loss(SSNHL) and therapeutic effect, and the ef?fect of glucocorticoid(GC)to expression of GR. Methods 196 patients with SSNHL were randomly divided into two groups:pa?tients in groups A were treated by prednisone and Ginaton, patients in groups B were only treated by prednisone. Patients were divided into responders and nonresponders according to therapeutic effect . The mRNA express of GRαand GRβin PBMCs of every patient was detected by real time quantitative RT-PCR on pretherapy and post-therapy.The difference of GRαmRNA , GRβmRNA and GRα/GRβamong groups and between pretherapy and post-therapy was analyzed. Results 150 patients with SSNHL were GC sensitive and 46 patients were GC resistan. On pretherapy, there were GRαand GRβmRNA in PBMCs of all patients, GRαwas visibly more than GRβ, there were not obvious difference of GRαmRNA ,GRβ mRNA and GRα/GRβ in PBMCs among responders and nonresponders between two groups . There was no statistical difference of GRαmRNA between responders and nonresponders in the same group. GRβmRNA of nonresponders was significantly more than that of responders(P<0.01), GRα/GRβof nonresponders was significantly lower than that of responders(P<0.01) . On post-therapy, there were also not obvious difference of GRαmRNA ,GRβmRNA and GRα/GRβamong responders and nonre?sponders between two groups. GRαmRNA ,GRβmRNA and GRα/GRβwere all significantly decreased in both groups than pretherapy(P<0.01 in responders, P<0.05 in nonresponders). Conclusion GRβmRNA in PBMCs of patients with SSNHL is inversely correlative with the hearing prognosis ,whereas GRαmRNA is independent on the hearing prognosis.GC can down-regulate GRαmRNA and GRβmRNA of patient with SSNHL. Ginaton has no effect to the expression of GRαand GRβmRNA of patient with SSNHL.