Effects of Ligustrazine on Expression of Fas/Fas L and Caspase8 in Cochlea of Rat with Cisplatin-induced Hearing Loss

LIU Hongwei; LENG Hui; SUN Haibo

doi:10.3969/j.issn.1672-2922.2015.02.035

Abstract：Objective The anti apoptosis effect of Ligustrazine against cisplatin induced hearing loss model rat cochlear hair cells, and to explore its anti apoptotic mechanisms. Method 120 SPF Wistar were divided into 4 groups, normal control group;normal+tetramethylpyrazine group;model group;model of Kagawa group. The model drug deafness rats induced by in?traperitoneal injection of cisplatin by intraperitoneal injection, ligustrazine intervention on cisplatin deafness model rat cochle?ar basilar membrane, method detection of brainstem auditory evoked potentials of ABR threshold and rat stretched the model was evaluated, and to detect the level of Fas, Fas L and caspase8 mRNA and protein expression in cochlear tissues of rats in each group. Results The model control threshold of ABR rats was significantly higher than that in normal control group, ligust?razine can decrease ABR threshold cisplatin deafness model rats;change of cisplatin in rat cochlea hair cells loss model was dissolved, disorganized, lack of Pathology;water were significantly higher than those in normal control group Fas, Fas L and cas?pase-8 mRNA and protein expression of the control group rat cochlear model by intraperitoneal injection, ligustrazine interven?tion, three expression level to be a certain control. Conclusions Cisplatin induced rat model of drug-induced deafness, ligust?razine has certain intervention effect on deaf rat model;intraperitoneal injection of cisplatin can result in cochlear tissues in rats Fas, Fas L and caspase8 mRNA and protein expression level, and ligustrazine injection may be to achieve its anti apoptotic effects through inhibition of Fas, Fas expression level L and caspase8 mRNA and protein.