Energy Metabolism Study of AIF Gene
YANG Ju
GUAN Jing
ZONG Liang
WANG Hongyang
ZAHNG Chao
ZHANG Yue
GUO Weiwei
ZHAI Suoqaing
WANG Qiuju
Abstract：Objective To know of the functional features of AIF gene in cell metabolism process, and to further ex-plore molecular mechanism of AIF related auditory neuropathy. Methods For the aim of obtaining AIF Knock out cell line, the Lipofectamine 2000 was used to transfect CRISPR-Cas9 in HELA cells. Western blot and Sanger sequence were per-formed to test protein expression and genotype analysis, respectively. Oxygraph-2K was chosen to detect the oxidation func-tion of multiple AIF knockout cell line and wild-type cell line, GraphPad prism5 was used to analyze data. Results Multiple strains of AIF gene knockout cell lines were obtained by the methods, and the oxygen consumption function of wild type cell lines were significantly stronger than the AIF gene knockout cell lines. Conclusion This study compared the oxygen con-sumption difference between AIF-deficient cells lines and wild-type cells lines by applying CRISPR-Cas9 technology, indi-cating that the protein expression of AIF plays a key role in maintaining cells energy metabolism, which may be an incentive mechanism of auditory neuropathy.
Keywords：AIF geneAuditory neuropathyCRISPR/Cas9KnockoutOxygen consumption
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 250-254 )