CRISPR/Cas Targeted Editing Technique System and Animal Model of Deafness
DUAN Hong
YUAN Huijun
Abstract：The CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system is a new generation of targeted genome editing technique system. Its prominent feature is the Cas protein, which is capable of targeted cutting of exogenous DNA under the guidance of gRNA (guide RNA) and provides immunity to bacteria invasion of nu?cleic acids. CRISPR/Cas system,mediated targeted gene modification technology has been successfully has been applied to a variety of bacteria and other biological genomes, as well as to human genomic DNA. Compared previous technology, the CRIS?PR/Cas system has shown a number of advantages on numerous aspects. The CRISPR/Cas system is now an important techni?cal means in establishing animal model of genetic defect diseases. Various types of genetically modified animal models are a preferred approach and essential in studying inner ear gene functions and molecular mechanisms of deafness. Deafness gene studies advance and diagnosis improves, there will be increasing needs for animal models of hereditary deafness. CRISPR/Cas system mediated targeted the genome modification technology will continue to improve and will play an increasingly important role in many fields of genetic research and diagnosis and treatment of deafness.
Keywords：CRISPR/Cas systemDeafnessAnimal model
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 171-175 )