Effect of Heme oxygenase-1 in spiral Ganglion Neurons Following Cisplatin-induced Injury in Guinea Pigs
DENG Dan
LUO Wenlong
Abstract：Objective To investigate effects of heme oxygenase-1(HO-1) on spiral ganglion cells neurons (SGNs) in cis?platin-induced injury. Methods Forty adult guinea pigs were divided randomly into 4 groups (n=8 in each group) to receive intraperitoneal injection of 0.9%saline (Group A), or cisplatin (Group B), or ferulic acid (150 mg/kg, bid) and cisplatin (Group C), or ferulic acid (150 mg/kg, bid), zinc-protoporphyrin-Ⅸ(15 umol/kg, bid) and cisplatin (Group D), all at 12 ml/kg, bid for 3 days. HO-1 expressing pattern in damaged cochlea was studied by immunochemistry and Western Blot techniques. Results in Group A, faint HO-1 expression was seen in the spiral ganglion tissue with its protein expression only at 0.48 ± 0.07. HO-1 protein expression was 0.81 ± 0.07 in Group B, 0.9 ± 0.05 in Group C and 0.61 ± 0.07 in Group D. The differences in HO-1 pro?tein expression between the four groups were statistically significant (p<0.0001). Conclusion Among animals exposed to cispl?atin, expression of HO-1 was the highest in Group C and the lowest in Group D, indicating possible HO-1 involvement in the re?pair process in spiral neurons following damage by cisplatin.
Keywords：HO-1Ferulic acidGuinea pigSpiral neurons
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 156-160 )