Protective Effects by Water Extracts of Herba Artemisiae Capillariae Against Bilirubin Induced Neuroblastoma Apoptosis(SH-SY5Y)
ZHANG Yi
LIANG Yong
LI Peng
FU Qiuyang
LIU Zhao
LI Qi
ZHANG Wei
YANG Chen
Abstract：Objective To investigate the mechanism of protection by capillary artemisia aqueous extract (Yincheng ex?tract, YCE) against bilirubin induced neuroblastoma apoptosis. Methods MTT was used to assess SH-S5Y5 cells prolifera?tion. Western blotting was employed to examine NF-KB protein expression in the nucleus and cytoplasm. Changes of JC-1 probe labeled mitochondrial potential were examined by immunofluorescence microscopy. Annexin-FITC/PI was used to detect cell apoptosis with flow cytometry. IL-1β and TNF- alpha changes were tested using quantitative fluorescence PCR. Results YCE improved concentration and time dependent inhibition of bilirubin’s effects on cell proliferation (p<0.05). YCE inhibited NF-KB nuclear transfer that was promoted by bilirubin. On immunofluorescence microscopy, mitochondrial mem?brane potentials decreased significantly in the bilirubin treated group compared with the control group (p<0.05), consis?tent with early apoptosis, which was seen reversed by YCE treatment (p<0.05). Flow cytometry annexin-FITC/PI results showed that bilirubin induced apoptosis was countered by YCE. Fluorescence quantitative PCR showed that the expression of inflammation-related proteins IL-1 beta and TNF- alpha (regulated by the transcription factor NF-KB) was decreased by YCE (p<0.05). Conclusion While bilirubin can induce apoptosis of SH-S5Y5 cells, YCE can effectively reduce NF-KB acti?vation and mitochondrial membrane potential changes, and thus inhibit apoptosis induced by bilirubin at multiple target points.
Keywords：Yincheng extractYCEBilirubinNeuroblastomaAapoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 151-155 )