Technical Details of Sample Preparation for Inner Ear Organotypic Cultures
YU Jintao
DING Dalian
LI Peng
GAO Kelei
JIANG Haiyan
SUN Hong
Richard J Salvi
Abstract：In vitro culture techniques have important values in the investigation of inner ear cellular functions, organic development, cell degeneration and/or cell regeneration. Since structures of the inner ear are tiny and complex, sample prepara?tion for organotypic culture of the inner ear can be difficult, despite that relevant techniques have been introduced briefly in numerous earlier publications. Based upon decades of culturing practices with inner ear end-organs in this lab, this article ad?dresses the technical aspects of inner ear organic culturing by firstly presenting a brief review of various culture methods for different inner ear materials. Detailed step by step protocols for sample preparation, collection and culture are also described. This presentation provides detailed operative guidelines regarding useful techniques and tricks in micro-dissection of the whole membranous labyrinth, in sample preparation, collagen gel preparation and sample layer mountings with detailed illus?trations. We hope this article can serve as an additional reading material supplementing previous literatures that lack detailed technical information for beginners in this field.
Keywords：Organotypic cultureRatCochleaVestibular organHair cellSpiral ganglion neuron
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 64-70 )