Establishment of an Animal Model of Laser Otomicrosurgery

TIAN Tianjie; YE Qing

doi:10.3969/j.issn.1672-2922.2014.04.042

Abstract：Objective The aim is to determine appropriate anesthetic and surgical approaches and optimal type of laser in an animal model of laser otomicrosurgery. Methods Seventy male guinea pigs with normal hearing were divided into groups A, B and C (n=20 in groups A and B,and n=30 in group C). Animals received anesthesia with either SU MIAN XIN (Group A), ketamine (Group B) or compound anesthesia (Group C). The survival rate was compared between groups, as well as the anesthesia recovery time of ten guinea pigs in each group. The bulla was opened to expose the basal turn of the cochlea and round window niche via a postauricular approach parallel to the external auditory canal. Fenestration on the basal turn was performed with CO2 laser. Results Group C showed the highest survival rate(p<a′ 0.0125)and shortest anesthesia recov?ery time(31.50±7.47min). The postauricular approach resulted in minimal tissue damage and bleeding, and led to easy expo?sure of the basal turn and round window niche,adequate for fenestration in the basal turn CO2 laser. Conclusions In establish?ing this animal model of laser otomicrosurgery, compound anesthesia appears to be the safest anesthesia approch, with a post?auricular approach parallel to the external auditory canal providing a perfect approach for operation, CO2 laser has become the focus of laser otomicrosurgery in recent years.