Preparation of An RNA Probe for In Situ Hybridization of Chicken Bmp4 and Cath1
TANG Lichuan
ZHANG Yue
ZOU Yihui
Abstract：Objective To report a method for preparation of RNA probes for detection of Chicken Bmp4 and Cath1 in si-tu hybridization. Method Plasmids carrying specific sequences of Bmp4 and Cath1 genes were amplified using the Plasmid Mini Kit, linearized by restriction enzymes, extracted and purified by phenol-chloroform, and then synthesized to form anti-sense probes with T3 RNA polymerase in vitro. Quality of the prepared probes was tested by agarose gel electrophoresis and in situ hybridization in chicken. Results Well purified Bmp4 and Cath1 RNA probes with satisfactory results in in situ hybrid-ization were confirmed. Conclusions The reported method provides an excellent way to prepare RNA probes for chicken Bmp4 and Cath1 for in situ hybridization, which is economic, effective, with great results and excellent quality.
Keywords：Probe preparationIn situ hybridizationBmp4Cath1Chicken
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 527-529 )