Blocking NMDA Receptors for Reversal of Excitotoxicity Processes in Spiral Ganglion Neurons Induced by Sodium Salicylate
LIU Yuan
YIN Shihua
ZHOU Qi
HUANG Xunjian
SHU Jingcheng
WEI Shunlian
Abstract：Objective To investigate the antagonism of non-specific blockage of NMDA receptors on the process of exci-totoxicity in spiral ganglion neurons induced by sodium salicylate. Methods Cultured spiral ganglion cells were divided into three groups on the basis of the drugs which were mixed into the culture medium:1 mM glutamic acid (the GLU group), 1 mM glutamic acid + 5 mM sodium salicylate (the salicylate group), or 1 mM glutamic acid + 5 mM sodium salicylate + 50 μM MK-801 (the MK-801 group) . After 24 hours of culturing and 3 hours of reaction with the agents, cells were collected and test-ed for transcription and expression levels of BDNF exonⅣ, BDNF exonⅥand Caspase-3 using real-time PCR and immuno-fluorescence. Results Compared with the GLU and MK-801 groups, the transcription of BDNF exonⅣ, BDNF exonⅥand Caspase-3, as well as the expression of Caspase-3, in the salicylate group were upregulated (p<0.05). Compared with the GLU Group, the transcription of BDNF exon Ⅳin the MK-801 group decreased (p<0.05). Transcription of BDNF exon Ⅵshowed no difference between the MK-801 and GLU groups, although there was in increase in expression of Caspase-3 (p<0.05). Conclusions Non-specific blockage of NMDA receptor can reverse the process of excitotoxicity in spiral ganglion neu-rons induced by sodium salicylate.
Keywords：NMDA receptorMK-801Sodium salicylateCochlearSpiral ganglion neuron
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 316-319 )