MicroRNA182 in Promoting In Vitro Cochlear Progenitor Cells Differentiation
XING Wei
YAN Hui
MI Wenjuan
CHEN Jun
QIU Jianhua
Abstract：Objective To investigate the effects of miRNA182 in promoting differentiation of cochlear precursor cells. Methods Cochlear precursor cells were dissociated and cultured. The cultured cells were divided into three groups: (1) the control group;(2) the miRNA182 mimics group;and (3) the miRNA182 inhibiter group. To analyze the process of precursor cells differentiation, cells collected 7 days after culture were run through flow cytometry for myosinⅦ+cells. The expression of SOX2 was analyzed by Western-blotting. Results More myosinⅦ+ cells were observed when precursor cells were cultured with miRNA182 mimics than others. The expression of SOX2 was significantly suppressed in the miRNA182 mimics group. Conclusion Our findings suggest that miR-182 can promote in vitro cochlear progenitor cell differentiation. Sox2 may be one of the target genes during mir-182 induced process, providing new target for hair cell repairing.
Keywords：miRNA182Cochlear progenitor cellsDifferentiationHair cell
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 312-315 )