A Preliminary Study of in Vitro Chondrogenic Potential of Human CD61+Adipose Derived Cells
Hengyun Sun
Guangdong Zhou
Yilin Cao
Abstract：Objectives To sort out chondrogenic subpopulation of human adipose derived cell (ADCs) with flow cytome-try and preliminarily examine its in vitro chondrogenic potential. Methods ADCs were isolated by enzymes. CD61+ and CD61- subpopulations were sorted out with flow cytometry and chondrogenically induced in vitro. After 3-week induction, col-lagen II, the specific extracellular matrix of cartilage, was examined with immunofluorescent staining and SOX9 and COL II, the specific genes of chondrocyte, were checked with PCR. Results CD61 could be used as a surface marker for cell sorting of ADCs with flow cytometry. The results of immunofluorescent staining demonstrated that after chondrogenic induction, both of the two cell populations expressed collagen II. Quantitative PCR results showed that the expression of SOX9 and COL II were higher in CD61+group than in CD61- group (p<0.05). Conclusions CD61+ADCs had a stronger chondrogenic differentiation capacity than CD61- cells. CD61 could be used as a specific marker for purifying chondrogenic subpopulation of ADCs.
Keywords：Chondrogenic subpopulation of ADCsCD61sorting out
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 540-542 )