Effects of Ouabain Concentration on Inner Ear Morphology and Hearing Thresholds in Guizhou Miniature Pigs
ZhangXue-Ru
Yang Shi-Ming
Li Deng-Ke
Ma Yue-Ying
Zhao Li-Dong
GuoWei-Wei
XingGuang-Qian
Abstract： Objective To investigate the relationship among cochlear morphology, hearing changes and ouabain concen-tration. Method Thirteen healthy, 2-months-old black Guizhou miniature pigs were randomly divided into an experimental group, an experiment control group and a normal control group. Ouabain (200 ul at 1 mM or 0.1 mM) was delivered through in-tact round window membrane. The same volume of saline was administrated to the experiment control group animals through the same pathway. No treatment was applied to the normal control group. Auditory brainstem responses were measured before and after ouabain delivery. The number of spiral ganglion neurons was counted under a microscope. The morphology of hair cells in the organ of Corti was examined after the application of drugs for three days, one week or two weeks. Results There was no significant changes in the morphology of the inner ear and auditory function in the experiment control and normal con-trol groups. After application of ouabain, the number of spiral ganglion cells decreased and the ABR threshold increased signif-icantly (P<0.05). The number of hair cells showed no significant change. Conclusion The spiral ganglion neuron can be in-jured and hearing threshold elevated by ouabain delivered through intact round window membrane in a dose and time depen-dent manner. Hairs cells are not significantly affected by ouabain.
Keywords：Inner ear hair cellsSpiral ganglion neuronAuditory neuropathyAnimal model
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 279-283 )