Based on UPLC-MS and Network Pharmacology to Explore the Potential Pharmacodynamic Substances of Shenqi Buqi Granules in the Treatment of Qi Deficiency Syndrome via"Treating Different Diseases with the Same Method"

QI Yutong; MIAO Lan; PENG Qing; MENG Shuo; LIU Jianxun

doi:10.3969/j.issn.1006-3250.2026.02.010

Abstract：Objective To explore the common material basis and molecular mechanisms of Shenqi Buqi granules for treating chronic atrophic gastritis,glomerulonephritis,obstructive pulmonary disease,liver cirrhosis,and heart failure as"treating different diseases with the same method"through component analysis coupled with network pharmacology and molecular docking.Methods Using hypoxic tolerance and fatigue resistance as evaluation criteria for improving Qi deficiency syndrome,the normobaric/hypoxic and weight-bearing swimming experiments were operated.The therapeutic effects of Shenqi Buqi granules on Qi deficiency were evaluated by comparing the duration of hypoxia tolerance and weighted swimming among mouse groups.Qualitative analysis of the major chemical components was identified using UPLC-Q/TOF-MS.The network of"chemical components-treating different diseases with the same method targets"was conducted,and core components and targets were identified through topological parameter analysis.AutoDock Tools was ultimately employed to validate the docking of the active ingredients with the targets.Results Pharmacological efficacy experiments showed that,compared with the normal control group,the survival time and weight-bearing swimming time of mice in the treatment group under normobaric hypoxia conditions were both prolonged,indicating that Shenqi Buqi granules have significant hypoxic tolerance and anti-fatigue effects.A total of 44 chemical components were identified in the compound,including saponins,flavonoids,nucleosides,etc.A total of 72 core targets related to Shenqi Buqi granules"treating different diseases with the same method"were screened out,finding 23 important components such as Ononin,Cyclosin,Ginsenoside Rg1 and Methylnissolin,as well as key target points such as AR,IL2,VEGFA,and EGFR.These targets were involved in regulating biological processes such as cell proliferation and apoptosis,and were associated with signaling pathways including AGE-RAGE,PI3K-Akt,TNF and HIF-1,and so on.Conclusion This study elucidates how the Shenqi Buqi granules exert therapeutic effects on five diseases as"treating different diseases with the same method"through a combined action involving multiple components,targets and pathways,providing a theoretical foundation for further development and application of Shenqi Buqi granules.