Exploring the Clinical Pathogenesis of Cerebral Infarction with Qi Deficiency and Blood Stasis Syndrome Based on Proteomics
LI Ying
ZHAG Guoyuan
SUN Mingqian
MIAO Lan
LIN Li
LIU Jianxun
Abstract：Objective To explore the biological basis of cerebral infarction(ischemic stroke)with Qi deficiency and blood stasis syndrome(CI-QDBS)by using proteomic techniques.Methods 24 patients with CI-QDBS were selected as the research group,and 24 patients with non-Qi deficiency and blood stasis syndrome and non-cerebral infarction were selected as the control group.Label free and LC-MS/MS techniques were used to perform quantitative proteomic analysis on plasma samples,and bioinformatics methods were used to screen for differential proteins and analyze the pathways involved.Results A total of 178 differentially expressed proteins were identified between the research group and the control group(113 up-regulated and 65 down-regulated),mainly enriched in platelet activation,complement and coagulation cascade,PI3K-Akt signaling pathway,etc.Key proteins include FGB,FGG,F2,F13A1,COL1A1,ITGA2B,etc.,which participate in the pathological process of CI-QDBS by regulating blood hypercoagulability,thrombus formation,and platelet activation.Clinical indicators showed that the research group had significantly increased systolic blood pressure,uric acid,triglycerides,and low-density lipoprotein(P<0.05),while high-density lipoprotein were significantly decreased(P<0.05),which were correlated with syndrome score and degree of neurological dysfunction.Conclusion The mechanism of Qi deficiency and blood stasis syndrome in cerebral infarction is closely related to the abnormalities of complement and coagulation cascade,and platelet activation pathway.Among them,platelet activation is the core link connecting molecular abnormalities and syndrome manifestations,providing a basis for research on the mechanism of platelet protein changes.
Keywords：Cerebral infarctionQi deficiency and blood stasis syndromeProteomicsComplementCoagulation cascadePlatelet activation
Publication Date：2026-02-28
Online Publishing Date：2026-03-13(First online date of this platform, not the publication date of the document)
Pages：6( 277-282 )