Mechanism of Naoshuantong Capsules Intervention in Acute Ischemic Stroke Based on GC-MS Metabolomics
XU Rufan
ZHANG Hongrui
LI Na
SONG Ke
XU Yihe
GAO Yonghong
LIU Zhenhong
GAO Ying
Abstract：Objective To investigate the protective effect of Naoshuantong capsules on acute ischemic stroke and its metabolic pathways and mechanism.Methods Mouse model of middle cerebral artery occlusion was established by the suture-occluded method.The mice that underwent successful model establishment were divided into the model group,the Low-dose Naoshuantong group,the High-dose Naoshuantong group and the nimodipine group.The sham group only underwent vascular operation separation.After three days,HE staining was used to observe the morphological structure of brain tissue cells.GC/MS metabolomics technology was used to detect endogenous metabolite changes in mouse brain tissue and then performed statistical analysis,the metabolomics detection additionally set up a sham surgery group(Sham),a model group(Model),and a Naoshuantong capsules group(NSTC),screened differential metabolites between groups,and conducted metabolic pathway enrichment analysis.Results HE staining revealed that mice in the NSTC-L and NSTC-H groups exhibited milder structural disorganization and relatively normal nuclear morphology,the brain tissue injury was improved compared to the model group.Metabolomic analysis was performed on the NSTC group and the Model group.20 differential metabolites were screened out between the two group,6 metabolites were upregulated,including adenosine,oleamide,and N-acetylaspartic acid,et al.14 metabolites were downregulated,including N-methylalanine,glycyltyrosine,and oleic acid,et al.It was concluded that the main metabolic pathways involved in the intervention of acute ischemic stroke by Naoshuantong capsules included cAMP signaling pathway,Glyoxylic acid and dicarboxylic metabolism,Synthesis and degradation of ketone bodies,cGMP-PKG signaling pathway,Vascular smooth muscle contraction,Regulation of lipolysis in adipocytes,Sphingolipid signaling pathway.Conclusion Naoshuantong capsules can improve brain injury in MCAO mice through metabolic pathways such as cAMP signaling pathway,cGMP-PKG signaling pathway,Synthesis and degradation of ketone bodies,Regulation of lipolysis in adipocytes and Sphingolipid signaling pathway.
Keywords：Naoshuantong capsulesAcute ischemic strokeMetabolomicsMCAO model
Publication Date：2026-02-28
Online Publishing Date：2026-03-13(First online date of this platform, not the publication date of the document)
Pages：8( 255-262 )