Analysis of the Mechanism of Jianpi Bushen Formula in Improving Aging Leydig Cells and Testosterone Secretion Based on the"Reproduction-Stimulating Essence"Theory
SUN Jie
GUO Yang
MA Yong
LI Muzhe
XUE Chunchun
LI Xiaofeng
XUE Yongpeng
YANG Jiafan
DA Weiwei
SHI Qi
TANG Dezhi
Abstract：Objective To investigate the mechanism of Jianpi Bushen formula(JPBSF)in improving aging Leydig cell models.Methods A D-gal-induced aging Leydig cell model was established and divided into four groups:normal Leydig cells(control),aging Leydig cells(model),blank serum,and drug-containing serum.Testosterone secretion was measured by ELISA;cell proliferation activity was assessed using CCK-8 assay;cell aging was observed via β-galactosidase staining;and gene/protein expression related to aging and testosterone synthesis was detected by RT-qPCR and Western blot.Results Compared with the control group,the model group showed significantly reduced testosterone secretion and cell proliferation(P<0.01),along with increased β-galactosidase-positive cells and upregulated p21/p53 gene expression(P<0.01).In contrast,the drug-containing serum group exhibited higher testosterone levels and cell proliferation(P<0.01),reduced aging cells(P<0.05),downregulated p21/p53(P<0.01),and upregulated expression of testosterone synthesis proteins(StAR,3β-HSD,SF-1)at both mRNA and protein levels(P<0.05).Conclusion JPBSF effectively ameliorates cell aging and enhances proliferation,likely by activating testosterone biosynthesis-related enzymes,thereby restoring testosterone secretion.This study provides insights into anti-aging strategies for male reproduction and systemic aging based on the"Congenital and Acquired Essence"and"reproduction-stimulating essence"theories.
Keywords：Reproduction-stimulating essenceJianpi Bushen formulaAgingLeydig cellsTestosterone
Publication Date：2025-09-28
Online Publishing Date：2025-09-30(First online date of this platform, not the publication date of the document)
Pages：7( 1568-1574 )