Research on the Role of Cilaobao in Preventing and Treating Musculoskeletal Atrophy

ZHAI Jinna; CHE Yuexia; CHEN Yue; SUN Jiuxu; PEI Lingpeng

Abstract：Objective By observing the effect of Cilaobao on the content of bone and skeletal muscle and the expression of skeletal muscle protein and bone metabolism-related protein in rats with musculoskeletal atrophy,the therapeutic effect of Cilaobao on musculoskeletal atrophy was investigated.Methods Eighteen SD rats were randomly divided into blank group,model group and traditional Chinese medicine(TCM)group.Castration surgery was performed in the model group and the group,and dexamethasone sodium phosphate was injected intraperitoneally 1 week after surgery for 2 weeks.After 1 month of model establishment,rats in the TCM group were intragastrically administered with decoction of root bark of Cilaobao.After intragastric administration for 3 months,the rats in each group were examined for body composition,femoral bone mineral density,and ELISA was used to detect the contents of muscle atrophy box F protein(MAFbx)and muscle-specific ring finger protein-1(MuRF1)in the supernatant of skeletal muscle tissue,and the contents of osteocalcin targama-glaprotein(BGP)and tartrate resistant acid phosphatase 5b(TRACP-5b)in the serum;HE staining was used to observe the pathological morphology of spleen and kidney tissues.Results Compared with the blank group,the skeletal muscle mass index and bone mineral density decreased in the model group;the contents of MAFbx and MuRF1 in the skeletal muscle increased significantly(P<0.05);the serum BGP and TRACP-5b increased significantly(P<0.001);in the HE staining,the white pulp area of the spleen tissue became larger,the lymphoid nodules increased greatly,the mesangial matrix proliferated in the glomeruli,and the mesangial cells increased in the model group.Compared with the model group,the skeletal muscle mass index and bone mineral density increased,the contents of MAFBX and MuRF1 in skeletal muscle decreased significantly(P<0.05),the serum BGP and TRACP-5b decreased significantly(P<0.01),and the structure of spleen and kidney tissues in the TCM group was improved in HE staining.Conclusion Cilaobao has the effect of preventing and treating musculoskeletal atrophy,inhibiting skeletal muscle protein degradation,improving bone metabolism,and relieving the occurrence and development of musculoskeletal atrophy.