Establishment and Evaluation on Precancerous Lesions of Gastric Cancer Disease-Syndrome Combined Rat Model

ZHU Wanhong; GONG Yucheng; LI Yuan; JIA Yuanping; YAN Yinan; DING Xia

Abstract：Objective To establish a rat model of precancerous lesions of gastric cancer(PLGC)combined with disease and syndrome,and to provide a stable and reliable animal model in line with the characteristics of clinical diseases for the basic research of PLGC.Methods A total of 30 Wistar rats aged 3～4 weeks,half male and half female,were randomly divided into normal group and model group.The four-factor comprehensive modeling method was used,combined with 120 mg/L N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)free drinking,0.05%ranitidine feed free diet,2%sodium salicylate gavage,hunger and satiety disorders,continuous 32 weeks to establish PLGC disease-syndrome combined rat model.During the modeling period,the general conditions of the rats were dynamically observed.After the modeling,the syndrome characterization,gastric mucosal appearance and histopathological morphology,gastric pH and hematological indexes of the rats were comprehensively evaluated.Results The general condition of rats showed that since the second week of modeling,the body weight was lower than that in the normal group(P<0.05).The evaluation of syndrome characterization showed that at the 32nd week of modeling,the activity of rats in the model group decreased(P<0.01),irritability(P<0.05),pale eyes,ears and tongue(P<0.01),and thin claw nails(P<0.01).The appearance and histopathological morphology of gastric mucosa showed that the overall color of the whole stomach was light,the fore stomach was bulging,the color of gastric mucosa was light,the mucosal folds were reduced,and erosion and vegetations(P<0.01)were visible.The glandular structure and cell dysplasia were observed under the microscope,and the pathological diagnosis was confirmed as dysplasia.The results of pH test showed that the pH level in the stomach increased(P<0.05).Hematological tests showed that the whole blood low shear reduction viscosity and the whole blood high shear reduction viscosity increased(P<0.05),the red blood cell level and the hemoglobin level decreased(P<0.01),while the platelet level increased(P<0.05).Conclusion The four-factor comprehensive modeling method can be used to construct a PLGC disease-syndrome combined rat model in line with clinical characteristics for 32 weeks,which has the syndrome manifestations of Qi deficiency,blood stasis,Yin deficiency and blood deficiency.