Investigation of the Mechanism of Gegen-Danshen Drug Pair in the Treatment of Ischemic Stroke Based on Network Pharmacology and Experimental Verification
SUN Xiaoxin
WANG Ruihai
MIAO Qing
LIU Limei
Abstract：Objective The mechanism of Gegen-Danshen drug pair in the treatment of ischemic stroke(IS)was investigated by network pharmacology and experimental verification in vivo.Methods IS rat models were established by MCAO method,and administered with Gege,Danshen,low dose of Gegen-Danshen drug pair and high dose of Gegen-Danshen drug pair for 1 week.The infarction area was measured by TTC staining.The potential targets of Gegen-Danshen drug pair in treating IS were obtained by TCMSP,GeneCards and other databases.GO functional enrichment analysis and KEGG pathway enrichment analysis were performed.The predicted pathway was preliminarily verified by Western blot.Results Through network pharmacological analysis,66 potential active components of Gegen-Danshen drug pair were obtained.The key components were daidzein,luteolin,puerarin,salviolone,beta-sitosterol and tanshinone IIA.The key targets were IL6,TP53,VEGFA,MAPK1 and TNF.The possible mechanism was related to PI3K-Akt signaling pathway,HIF-1 signaling pathway,IL-17 signaling pathway,TNF signaling pathway,VEGF signaling pathway and other signaling pathways.The experimental results showed that after the intervention of Gegen-Danshen drug pair,the cerebral infarction area of MCAO rats was significantly reduced(P<0.01),and the expression of p-PI3K/PI3K and p-Akt/Akt in the brain tissue of MCAO rats was significantly increased(P<0.05).Conclusion Gegen-Danshen drug pair could significantly reduce the cerebral infarction area and alleviate the brain injury,which may be related to the regulation of PI3K-Akt signaling pathway.
Keywords：Gegen-Danshen drug pairIschemic strokeNetwork pharmacologyPI3K-Akt signaling pathway
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 272-279 )