Therapeutic Effect of Qingmai Yin on Experimental Sjögren's Syndrome Mice and Its Impact on B cell Subsets

HAN Man; LI Yaqing; ZHU Xiaoxia; ZHANG Huadong; ZHAO Xueqi; JIANG Quan

Abstract：Objective To study the effect of Qingmai Yin on the treatment and B cell subpopulations in mice with experimental Sjögren's syndrome(ESS).Methods Twenty-four C57BL/6 mice were randomly divided into normal group,model group,low-dose group of Qingmai Yin(1.125 g/mL),and high-dose group of Qingmai Yin(2.25 g/mL),six mice in each group.The ESS mouse model was constructed by subcutaneous injection of salivary gland protein emulsion into the mice,and after successful modelling,the mice were gavaged continuously for 6 weeks.At the end of gavage,salivary flow was detected,and serum anti-SSA antibody and anti-M3R antibody levels were detected by ELISA.Changes in the size and morphology of the spleen and cervical lymph nodes of mice were observed,and single-cell suspensions of the spleen,cervical lymph nodes,and bone marrow of mice in each group were prepared,and the proportion of distribution of each B-cell subpopulation was detected by flow assay.Results Compared with the normal group,the saliva flow in the model group decreased(P<0.01),the spleen and cervical lymph nodes were significantly increased(P<0.01).The level of ant-ssa and anti-M3R antibody increased(P<0.05),the proportion of GCB cells in spleen and cervical lymph nodes increased(P<0.05),the proportion of plasma cells in spleen and bone marrow increased(P<0.05),the proportion of B cells in the marginal area of the spleen decreased(P<0.05),and the proportion of follicular B cells increased(P<0.01).Compared with the model group,the saliva flow in the Qingmai Yin intervention group increased(P<0.05),and the level of anti-M3R antibody decreased in the high-dose group(P<0.05).The level of anti-SSA antibody in the high-dose group was significantly decreased(P<0.01),the lymph nodes in the spleen and neck decreased to varying degrees(P<0.05),the proportion of B cells in the marginal zone of the spleen increased(P<0.01),and the proportion of follicular B cell subsets decreased(P<0.05)Conclusion Qingmai Yin may alleviate the dry symptoms of ESS,improve the antibody level,and mitigate the disease process of ESS mice by regulating the B cell subpopulations.