Analysis of Influence of Jiangxi Characteristic Processing Technology on Chemical Compositions of the Chinese Medicine Cimicifuga Rhizoma Based on UPLC-Q-TOF-MS
ZHU Jing
YUAN En
WU Yigeng
YI Bingxue
CHEN Qi
Abstract：Objective To compare the effects of different processing techniques on the chemical constituents of Cimicifuga Rhizoma for screening the dominant decoction pieces. Methods The chemical constituents of different processed products of Cimicifugae Rhizoma were analyzed by UPLC-Q-TOF-MS in positive and negative ion modes,and identified by reference substance,relative molecular mass,mass spectrometry fragmentation rule and literature information. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) models were developed for each processing of Cimicifuga Rhizoma using SIMCA-P13. 0 software to obtain PCA score plots,PLA-DA score plots and projection of variable importance (VIP) values to screen the material bases responsible for the major differences before and after processing of Cimicifuga Rhizoma. Using the MetaboAnatyst web mapping tool,a thermogram was produced to obtain a more visual observation of the trends in the chemical composition of Cimicifuga Rhizoma after processing. Results 71 chemical components were identified. PCA indicated that there were significant differences among different groups of Cimicifuga Rhizoma processed by different methods. A total of 33 chemical components with VIP>1 was screened by PLS-DA as the main chemical markers for the differences before and after processing. The content of triterpenes in raw products and honey fried Cimicifuga Rhizoma is higher,the content of phenolic acids in honey bran and honey chaff fried Cimicifuga Rhizoma is higher,and the content of ferulic acid in honey bran Cimicifuga Rhizoma is higher. Conclusion Phenolic acids and triterpene saponins are the most important compounds to distinguish different processed products of Cimicifuga Rhizoma,this paper provides a basis for the study of the pharmacodynamic material basis and advantageous varieties of Cimicifuga Rhizoma decoction pieces with Jiangxi characteristic.
Keywords：Cimicifuga RhizomaProcessingChemical constituentsUltra-high performance liquid chromatography-quadrupole-time of flight mass spectrometryPrincipal component analysisPartial least squares-discriminant analysisHeatmap
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1935-1941 )