Effect of Jingui Shenqi Pill on the Levels of Monoamine Neurotransmitters in Rats with Kidney Yang Deficiency Constitution

LIANG Xuanwei; LAI Han; WU Jing; WANG Yi; LI Han; LI Weihong

Abstract：Objective To study the changes in monoamine neurotransmitter levels in rats with kidney Yang deficiency constitution induced by low-temperature and cold food diet,and the regulatory mechanism of Jingui Shenqi pill. Methods The model of kidney Yang deficiency constitution in rats was prepared by administering 0. 54g/(kg·d) salted Cortex Phellodendri granules,exposure to an artificial cold environment of 4 ℃ for 1 hour per day,and passage. The model was maintained until the offspring rats were weaned,and the modeling continued until they were 12 weeks old. The offspring of the control group of the parent rats were the control group of the offspring,and the offspring of the model group of the parent rats were randomly divided into the model group and the intervention group of the offspring. The intervention group of the offspring was given 1.08 g/(kg·d) Jingui Shenqi pill intervention from 8 weeks of age. Male rats from each group were selected as the research subjects,with 6 rats in each group. Their behavioral changes were observed using an open field experiment and temperature preference test,and the levels of BDNF,TrkB,CREB,TRPC3,TRPC6,5-HT,DA,and NE in the hippocampus tissue or serum were detected using ELISA method. Results Compared with the control group,the model group showed significantly decreased distance and speed of movement in the open field experiment(P<0. 05),significantly increased immobility time(P<0. 05),and significantly increased hot plate stay time in the temperature preference test(P<0.05),as well as significantly increased levels of various molecular indicators above in hippocampus tissue or serum (P<0.05). Compared with the model group,the intervention group showed significantly increased distance and speed of movement in the open field experiment(P<0. 05),significantly decreased immobility time(P<0. 05),significantly decreased hot plate stay time in the temperature preference test(P<0.05),as well as significantly decreased levels of various molecular indicators above (P<0.05). Conclusion Jingui Shenqi pill can regulate the abnormally elevated levels of monoamine neurotransmitters caused by kidney Yang deficiency constitution. Its mechanism may be related to the BDNF/TrkB/CREB pathway and the classic transient receptor potential channel family represented by TRPC3/6.