Establishment of Blood Deficiency Syndrome Model in Bama Miniature Pig

LIU Chun; ZHU Keyu; DUAN Junguo

Abstract：Objective To establish a blood deficiency syndrome model in Bama miniature pig by using acetylphenylhydrazine(APH) in combination with cyclophosphamide(CTX). Methods Twelve Bama miniature pigs were randomly divided into model group and blank group,with 6 pigs in each group. After 7 days of routine feeding,pigs in the model group were injected subcutaneously with APH at a dose of 50 mg/kg on the first and fourth day,and then intraperitoneally injected with CTX at a dose of 20 mg/kg from the fifth to the eighth day to establish the blood deficiency syndrome model in Bama miniature pig. The general signs of the two groups of Bama miniature pig were observed,and the red blood cell count(RBC),mean corpuscular hemoglobin(MCH),hematocrit(HCT),hemoglobin(HGB),white blood cell count(WBC),number of monocytes(MON),lymphocyte number(LYM),and number of neutrophil(NEU) were measured to evaluate whether the model had been successfully established. Results The model group of Bama miniature pig exhibited signs of blood deficiency syndrome,such as lethargy,pale mucosa and limbs,and rough and dry hair,compared to the blank group. Additionally,the model group showed a decrease in RBC,HCT,HGB,WBC,MON,and LYM,with statistically significant differences (P<0. 05). Conclusion The method of subcutaneous injection of APH at a dose of 50 mg/kg on the first and fourth day,followed by intraperitoneal injection of CTX at a dose of 20 mg/kg from the fifth to the eighth day,can successfully establish a blood deficiency syndrome model in Bama miniature pig.