Study on the Effect and Mechanism of Huanglian-Sichuan Pepper Extract on Hyperlipidemic Mice
MIAO Qing
LIU Yanjun
WANG Ruihai
LIU Limei
HU Jingqing
Abstract：Objective To investigate the effect and possible mechanism of the compatibility of Huanglian-Sichuan Pepper extract on hyperlipidemia mice.Methods ApoE-/-mice were fed with high fat to establish hyperlipidemia model and treated with Huanglian-Sichuan Pepper extract.The serum levels of TC,TG,LDL-C,HDL-C,ALT,AST,TNF-α,IL-1β and IL-6 were detected by automatic biochemical analyzer.HE staining or oil red O staining were used to observe the pathological changes of liver tissue of mice.ELISA and RT-qPCR were used to detect the level and mRNA expression of HMG-CoA reductase in the liver.Western blot was used to detect the related protein expression of ERK/p38 MAPK signaling pathway.Results After administration,the levels of TC,TG,LDL-C,ALT,AST,TNF-α,IL-1β and IL-6 in the serum of mice were significantly decreased(P＜0.05),hepatocyte fat vacuoles and lipid deposition were reduced,and hepatic steatosis was alleviated,the activity and expression of HMG-CoA reductase in liver were significantly decreased(P＜0.01),the expression of p-ERK1/2/ERK1/2 and p-p38/p38 were significantly decreased(P＜0.01).Conclusion The compatibility of Huanglian-Sichuan Pepper extract can reduce the blood lipid level,alleviate hepatic steatosis of hyperlipidemia mice.It can still inhibit the hepatic HMG-CoA reductase to reduce cholesterol synthesis and inhibit the expression of inflammatory factors.The mechanism may be related with regulating the ERK/p38 MAPK signaling pathway.
Keywords：Cold and heat methodHuanglian-Sichuan Pepper extractHyperlipidaemiaHMG-CoA reductaseERK/p38 MAPK signaling pathway
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1715-1720 )