Reproduction and Evaluation of A Mouse Model of Rheumatoid Arthritis with Interstitial Lung Disease
SHANG Biyue
JIANG Quan
XIA Congmin
YAO Chuanhui
CHANG Tian
LIU Zixia
MA Xieli
YANG Yuchen
GONG Xun
HUANG Guangrui
Abstract：Objective The collagen-induced arthritis(CIA)model and the collagen-induced arthritis model combined with bleomycin-induced pulmonary fibrosis(CIA-BLM)mouse model were replicated and evaluated.Methods Fifteen DBA-1 mice were randomly divided into blank group(NC),model group(CIA),and composite model group(CIA-BLM),with 5 mice in each group.Paw swelling,Arthritis score(AI),HE staining,Masson staining,joint and lung imaging performance,and lung function were used to evaluate the model.Results Compared with the NC group,body weight,toe swelling and AI score of the rats in CIA and CIA-BLM group decreased(P<0.01).Compared with the NC group,the histopathology of the CIA group and the CIA-BLM group rats showed abnormal lung tissue structure,thickening of alveolar wall,inflammatory cell infiltration,and obvious blue collagen fiber deposition in the CIA-BLM group compared with the CIA group.Micro-CT showed that the joint damage was severe in rats of the two model groups.The imaging of the lungs in the CIA group showed local ground-glass manifestations,and in the CIA-BLM group,flocculent opacities,grid opacities,and bronchial traction dilation were the main manifestations.Lung function decreased in both model groups.Conclusion Both the CIA model and the CIA-BLM model had the characteristics of joint destruction and pulmonary fibrosis,and according to the results of histopathology,imaging and pulmonary function tests,the pulmonary fibrosis characteristics of the CIA-BLM model were more obvious.
Keywords：Rheumatoid arthritisRheumatoid arthritis combined with interstitial lung diseaseBleomycinAnimal model
Publication Date：2024-06-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 959-963 )