Yao Wei's Experience in the Treatment of Insomnia and the Potential Mechanism of the New Prescription

JU Wenxue; YAO Wei

Abstract：Objective Based on the traditional Chinese medicine inheritance assistance platform,to explore the medication patterns of Professor Yao Wei in treating insomnia and explore the mechanism of potential new prescriptions in treating insomnia.Methods 200 cases of insomnia in outpatient clinics were collected,and a database was established using traditional Chinese medicine inheritance assistance platform software.Prescription drugs were analyzed for four Qi and five flavors,meridian tropism,frequency statistics,formula formation rules,and potential new formulas.Professor Yao Wei's medication patterns and formula formation ideas were explored.The network pharmacology method was used to explore the Active ingredient,targets,and signal pathways of potential new prescriptions.Results A total of 59 prescriptions and 168 medicines were involved in the 200 prescriptions.The core prescription"Licorice-Pinellia ternata-Poria cocos-Fructus Aurantii-Codonopsis pilosula-Tangerine peel-Bupleurum chinense-Scutellaria baicalensis"was analyzed,and the potential new prescription"Muxiang-Bergamot-Atractylodes macrocephala-Patchouli-Qingpi-Myrobalan-Schisandra chinensis-magnolia officinalis"was selected for network pharmacology research.After screening,44 effective active ingredients were obtained,and 104 relevant action targets of"treating different diseases simultaneously"were obtained.The key active ingredients including Quercetin Naringenin,stigmasterol,Nobiletin,and iris can be screened from the protein interaction network,including IL-6,AKT1,TNF,and IL-1 β,VEGFA,CASP3,PPARG,CXCL8 and other target proteins with high frequency(all higher than 50),and GO function enrichment analysis and KEGG pathway enrichment analysis were carried out on the above key targets,it was found that the potential new treatment for insomnia was related to AGE-RAGE signal pathway,IL-17 signal pathway,tumor necrosis factor signal pathway,etc.Conclusion Professor Yao Wei believes that the core pathogenesis of insomnia is"spleen deficiency and liver stagnation,disharmony of Qi and blood,and restlessness of the mind and soul".The treatment focuses on regulating the Qi,regulating the essence of the spleen,and treating the heart and liver together.Potential new therapies may play a role in treating insomnia through the AGE-RAGE signaling pathway,IL-17 signaling pathway,tumor necrosis factor signaling pathway,etc.