A Novel Perfusion Fixation Device and Method Suitable for Morphological Study of Rat Myocardial Tissue
XIN Juanjuan
ZHANG Wenxi
LIU Qun
ZHAO Yuxue
ZHOU Chen
WU Shuang
ZHAO Chunxiao
ZHANG Ying
YU Xiaochun
GAO Junhong
Abstract：This article introduces an improved new experimental device and method suitable for perfusion sampling of rat myocardial tissue in the study of cardiovascular effect mechanisms.The innovation of this study is that it combines the principle of retrograde perfusion of the isolated heart aortic cannula with the traditional perfusion method,and the design of this new device can avoid the injury of rat heart from aortic clamp and needle during cardiac perfusion.Moreover,the device is simple and easy to operate.The myocardial tissue sections prepared by the new device are intact and beautiful,the myocardial tissue structure under the microscope is clear,and the fluorescent immunohistochemical staining effect is ideal.Through the application of this device and method,it is conducive for morphological observation and result display related to acupuncture,medicine or acupuncture combined with cardiovascular effect mechanism research.
Keywords：Myocardial tissuePerfusionFixationRatExperimental device
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2010-2013 )