Reevaluation of Rat Model of Blood Stasis Syndrome Induced by Carrageenan

LIU Yue; YAO Mingjiang; FU Guojing; FAN Xueming; DU Wanqing; CHI Xiansu; GAO Yunxiao; ZHANG Zhen; LIU Hongxi; LIU Jianxun; ZHANG Yunling

Abstract：Objective To establish an animal model of blood stasis syndrome and to construct a more systematic and comprehensive evaluation system for animal models of blood stasis syndrome.Methods SPF male SD rats were randomly divided into normal group and carrageenan(Ca)low,medium,and high dose(5,10,20 mg/kg)model group;the model group was injected with the corresponding dose of carrageenan in the peritoneum and the normal control group was injected with 0.9%sodium chloride solution.At 24 h and 48 h after injection,the general condition,black tail length,caudal blood perfusion,tongue,blood rheology,and platelet aggregation rate were observed and detected in each group of rats,and the tail tissues of each group of rats were retained for pathological section analysis.Results Rats in the medium and high-dose groups showed signs such as decreased activity,decreased eating,loose and dull fur,erect hair,and purple and dark auricular veins at 24 and 48 hours after molding.24 h after molding,the model low,medium,and high-dose groups all showed off black tails,and the length of the black tail in the high-dose group increased significantly compared with the low-dose group(P<0.01);48 h after molding,compared with the low-dose group,the length of the black tail in the medium-dose and high-dose groups increased significantly(P<0.01 or P<0.05).24 h and 48 h after molding,the tail-end blood flow of each dose group of the model group was significantly reduced compared with the normal group(P<0.01),and no significant difference was found between the model groups.In the medium-dose group and high-dose group of the model,the values of tongue images R,G,and B decreased significantly compared with the normal group 24h and 48h after molding(P<0.01 or P<0.05).The whole blood viscosity(low,medium,and high cut)was significantly increased in the medium-and high-dose groups at 24 and 48 hours after molding(P<0.01 or P<0.05).At 24 and 48 h after molding,the maximum aggregation rate of platelets in the medium-dose and high-dose groups was significantly higher than that in the normal group(P<0.01 or P<0.05).The result of tail histopathology showed that a small number of inflammatory cells were visible in low-dose groups.A large number of inflammatory cell infiltrates,interstitial edema,thin tube walls,and erythrocyte spillage could be seen in the medium-and high-dose groups.Conclusions The medium-dose(10 mg/kg)and high-dose(20 mg/kg)carrageenan-induced rat blood stasis model showed more stable and typical blood stasis symptoms at 24 h and 48 h after molding,and a model evaluation system involving macroscopic characterization of blood stasis syndrome,modern medical microscopic indicators and histopathological morphology of TCM was constructed in this study.