Effect of Yiqi Yangyin Qingre Huayu Recipe on Notch Signaling Pathway in Mice with Immune Thrombocytopenia
XIE Dong-jie
WANG Ai-di
WANG Wei-tao
ZHANG Le
ZHANG Shan
LIU Bao-shan
Abstract：Objective:To clarify the effect of Yiqi Yangyin Qingre Huayu Decoction for ITP and observe the influence for Notch molecular,exploring the potential mechanism for the treatment of ITP.Methods:60 mice randomly divided into normal group (n =10) and ITP model group (n =50),BALB/C mice were rendered thrombocytopenia by daily intraperitoneal injection of 2 pg rat anti-mouse CD41 monoclonal antibodies (MWReg30) in 200 μL phosphate-buffered saline (PBS) in model group.On the 8th day,After the evaluation,the ITP model mice were divided into different drug intervention groups which was performed with different drug interventions for 2 weeks,the platelet count was observed after treatment and then the gene and protein expression levels of Notch1-4 and Jagged1-2 in the bone marrow mononuclear cells (BMMNC) were measured by RT-PCR and Western blot respectively.Results:Each dose of Chinese medicine treatment group can significantly increase the platelet compared to model group and reduce notably Notch(1-4),Jagged1,2 gene and protein expression levels.Conclusion:Yiqi Yangyin Qingre Huayu Decoction plays a role in elevating platelet level by reducing the expression of Notch signaling to influence the pathology progress of ITP and achieves eventually the purpose of treatment of ITP.
Keywords：Yiqi Yangyin Qingre Huayu DecoctionImmune thrombocytopeniaNotch signaling pathway
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 38-41 )