Effect of inkgolide B on the Expression of Synapsin-1, Beclin1 and LC3 in Hippocampus of Alzheimer's Disease Rats
TIAN Xin-hong
HAO Li
YOU Yan-wen
XU Yu-ying
YU Shi-qi
Abstract：Objectivel:To evaluate the efficiency of Ginkgobalide B (GB) on the expression of Synapsin-1,Beclin1 and LC3 in the hippocampus of Alzheimer disease's rats (AD rats).Methods Rats were randomly divided into four groups:sham group,model group,three Ginkgobalide B groups of low dose,middle dose and high dose respectively.The Morris water maze (MWM) test was used to assess behaviors of the rats,Transmission electron microscope (TEM) was used to observe autophagy in hippocampal CA1 neurons of each group,Imunohistochemical staining was used to test Synapsin-1,Beclin1 and LC3 in the hippocampal CA1,Real-time PCR was used to examine Synapsin-1,Beclin1 and LC3-Ⅱ mRNA levels.Results:MWM test result showed that escape latency was significantly shower and the times of crossing the platform increased.GB could promote hippocampal CA1 neurons autophagy.Imunohistochemical staining result displayed that Synapsin-1 increased and Beclin1 decreased in the hippocampal CA1 after GB(5,7.5mg/kg)treatment.Real-time PCR manifested that compared with the model group,Synapsin-1 increased,Beclin1 and LC3-Ⅱ decreased in the hippocampus after GB(5,7.5mg/kg)treatment.Conclusion GB can improve learning and memory in AD rats,and its mechanism might be associated with Synapsin-1,Beclin1 and LC3.
Keywords：Ginkgobalide B(GB)Alzheimer disease(AD)Synapsin-1Beclin1LC3
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1701-1704,1759 )