Experimental Study on Apoptosis of Human Erythroleukemia Cell Line K562 Induced by Sinomenine

MOU Feng-lin; CHEN Hua

Abstract：Objective:To grope preliminarily the effects of Sinomenine(SIN) on the proliferation inhibition,apoptosis of human erythroleukemia cell line K562.Methods:CCK-8 assay was used to detect the K562 cell proliferation activity.K562 cell apoptotic changes were detected by Flow cytometry (FCM).The cell morphological changes of apoptosis were observed by hoechst staining.The changes of expressions of bcl-2 gene and bax gene were examined by Real-time PCR.The changes of expressions of Bcl-2、Bax and Caspase-3 proteins were examined by western blot analysis.Results:CCK-8 assay result showed that SIN can effectively inhibit the proliferation of K562 cells in vitro,which exhibits a dose-dependent manner.Flow cytometry results indicated that SIN can induce early apoptosis of K562 cells after induced by SIN,which showed statistically significant difference compared with the control group.Hoechst staining revealed that SIN could induce toypical apoptotic morphological changes.Real-time PCR showed that the expressions of bax gene was increased in dosedependent manner after induced by SIN,while expressions of bcl-2 gene were decreased compared with the control group.Western blot showed that the expressions of Bax,Caspase-3 were increased in dose-dependent manner after induced by SIN,expressions of Bcl-2 proteins were decreased compared with the control group.Conclusion:SIN can inhibit the proliferation of K562 cells and induce its apoptosis through activating Caspase-3 up-regulating the expression of Bax,and down-regulating the expression of Bax.