Differential Expression of Androgen Synthase in Different Syndromes of H22 Tumor Bearing Mice
PAN Zhi-qiang
FANG Zhao-qin
LU Wen-li
LIU Xiao-mei
LIANG Chao
ZHANG Yuan-yuan
Abstract：Objective:To study the relation of androgen synthases genes expression and syndromes in H22 tumor Mice.Methods:By the quantitative four diagnosis and syndrome differentiation methods,different H22 tumor mice with poisonous pathogenic factors syndrome and asthenia of qi syndrome in earlier period,asthenia of yang and qi syndrome on intermediate stage,asthenia of qi and yin and yang syndrome on advanced stage were obtained.Then testis cDNA microarray were detected by GeneChip Mouse Exon 1.0 ST Array.The androgen synthase and its regulatory factor genes expression were analyzed.Results:Compared with normal mice,Star,Stard5,Cyp17a1,Hsd3b6,Hsd17b3 and Sult1e1 genes expression were down-regulated in pathogenic factors syndrome and asthenia of qi syndrome on earlier period,and those genes were more significantly down-regulated in asthenia of yang and qi syndrome,asthenia of qi and yin and yang syndrome.Cyp11a1,Hsd3b1,Hsd17b10,Hsd17b11,Hsd17b12,Fdx1,Fdxr,Por and Sult1a1 genes expression were down-regulated only in asthenia of yang and qi syndrome,asthenia of qi and yin and yang syndrome.And Cyp17a1 specifically expressed only in testis.Conclusion:After the tumorigenesis,androgen synthases genes expression were decreased with spontaneous asthenia syndromes aggravation.
Keywords：Androgen synthaseSyndromeMiceGeneChipGenes expression
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1379-1383,1455 )