Effect of Spleen Deficiency 1 Prescription on Cytochrome C Oxidase Iv in Liver of Rats with Spleen Deficiency Type Functional Dyspepsia
LV Lin
WANG Feng-yun
TANG Xu-dong
MA Xiang-xue
YIN Xiao-lan
SHI Xiao-shuang
Abstract：Objective:To investigate the expression of COX Ⅳ protein and mRNA in Liver tissue of Dyspepsia rats with spleen deficiency type and the intervention by Pixu Ⅰ Recipe.Method:70 10-day-old male SD rat pups were randomly divided into normal group(n =10),FD model group (single model,n =10),FD with spleen deficiency model group (n =50).The normal control group were gavaged with 0.2 mL of 2％ sucrose,meanwhile,the FD with spleen deficiency model group were gavaged with 0.2 mL of 0.1％ IA in 2％ sucrose,for 6 days.To postnatal 43 day,the FD with spleen deficiency model group was given modified multiple platform method(MMPM),continued 14h every day,for 14 days.The FD with spleen deficiency model group were averagely randomly divided into double model group,domperidone group,low-dose,medium-dose and high-dose Chinese herbs group,and treated by drug for 14d.To detect the COX Ⅳ protein expression in liver tissues by immunohistochemistry,Western-blot and RT-PCR.Results:Compared with the normal group,the mRNA and protein expression of COX Ⅳ of double model group were significantly decreased;Compared with the double model group,the mRNA and protein expression of COX Ⅳ of high-dose Pixu Ⅰ Recipe group were increased.Conclusion:The FD Rats with Spleen Deficiency had the phenomenon of low expression of COX Ⅳ mRNA and protein,and Pixu Ⅰ Recipe could increase the expression of COX Ⅳ protein and mRNA,and then to promote metabolic energy.
Keywords：Spleen deficiency syndromeFunctional dyspepsiaCytochrome c oxidaseTraditional Chinese medicine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1234-1237,1252 )