Exploration of Pu Ren Dan Regulation Of Ob/Ob Signal Transduction Mechanism of Mouse Pancreatic Tissue
CHEN Shu
SULENG-GAO-WA
ZHANG Yao-dan
Bai Ying-hui
LU Bi-nan
Pang Zong-ran
Abstract：Objective:Phospho Explorer Antibody Microarray was applied to explore the signal transduction regulation mechanism of Pu-Ren-Dan in ob/ob mice pancreas.Method:Setting the obesity and insulin resistant ob/ob mice as model.Pancreas from the blank group,the model group and Pu-Ren-Dan group were detected by Phospho Explorer Antibody Microarray.Result:Compared with model group,61 different phosphorylated proteins were found in Pu-Ren-Dan group,which the variation trend was consistent with the blank group.Through the biological function analysis,the found 61 proteins were mainly involved in cellular process,response to stimulus,metabolic process,biological regulation,developmental process and immune system process.The Pathway-Act-Network was built according to the interactions with pathways identified in the KEGG database,including insulin signaling pathway,mTOR signaling pathway,MAPK signaling pathway,apoptosis,calcium signaling pathway etc.Conclusion:This may be one of the molecular mechanisms which PuRen-Dan intervenes in Pre-diabetes to regress the disease.
Keywords：Pre-diabetesPu-Ren-Danreversible phosphorylationsignaling pathway regulation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1229-1233 )