Study on the Cytological Mechanism of the Treatment of EGFRI Associated Eruption with Xiao Zhen and Antipruritic Spray
CHENG Zong-qi
CHEN Lin
YAO Xin
JIN Yi
Abstract：Objective:To explore the effect of Xiaozhen Zhiyang Spray on keratinocyte proliferation and differentiation and the effect on macrophage infiltration.Methods:MTT method was used to observe the different concentrations of Xiaozhen Zhiyang spray on skin keratinocytes HaCaT growth effect;Wound healing and Transwell experiment was used to examine the effect of Xiaozhen Zhiyang Spray on HaCaT and macrophage RAW264.7 exercise ability;Western blotting experiment was used to investigate the effect of Xiaozhen Zhiyang Spray on the proteinic expression of PI3K,AKT,tPA and CD147 in HaCaT cells and MMP-2,MMP-9 in RAW264.7 cells.RT-PCR was used to investigate the effects of Xiaozhen Zhiyang spray on MMP-2 and MMP-9 mRNA levels in RAW264.7 cells.Results:Xiaozhen Zhiyang Spray can dose dependently promote the proliferation of HaCaT cells and inhibit its differentiation.In wound healing and transwell experiment,the migration number and invasive ability of HaCaT and macrophage RAW264.7 decreased with Xiaozhen Zhiyang Spray concentration increased.Xiaozhen Zhiyang Spray can also dose dependently inhibit the expression of proliferation protein PI3K and AKT,and decrease the expression of differentiation protein tPA and CD147.In addition,Xiaozhen Zhiyang spray can significantly inhibit the mRNA and protein expression of MMP2 and MMP9 in RAW264.7.Conclusion:Xiaozhen Zhiyang spray can promote keratinocyte proliferation and reduce the infiltration of macrophage,which is one of the important mechanisms for the treatment of EGFRI associated skin rashes.
Keywords：Xiaozhen Zhiyang SprayHaCaTRAW264.7Proliferation and differentiationMigration and invasion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 1078-1084,封4 )