Effect of Replenishing Spleen Qi Deficiency Phlegm Method on AQP in the as Kidney Tissue

SHI Yue-ping; JIA Lian-qun; SONG Nan; LIU Jing-jing; YANG Guan-lin

Abstract：Object:To observe the changes of kidney water channel protein and cAMP/PKA signal pathway with the spleen dificiency and phlegm atherosclerosis bana mini-pig model and effect of the replenishing spleen on the model.Method:Fifteen bama minipigs were randomly divided into normal group,model group and replenishing spleen group with five in each.The spleen dificiency and phlegm atherosclerosis model were instant by high fat diet combined tied by running after balloon injury in the coronary artery.The bama minipigs of normal group were fed normal basic diet for 48 weeks,model group were fed high fat and high calorie fodder for 48 weeks.The bana mini-pigs of replenishing spleen group were gived replenishing spleen recipe on the basis of the method on model group after 24 weeks.Observing Cyclic adenosine monophosphate(cAMP) 、morphology and the protein expression of aquaporins (AQP)1、2、3 、phosphorylation protein kinase A(p-PKA) 、phosphorylated cAMP-response element binding protein (p-CREB) in the mini-pigs kidney after 48weeks.Result:Compared with normal group,morphology of kidney of the model group show the pathological damage in the glomerular and tubular,expression of AQP1、2、3 and cAMP/PKA/CREB pathway were reduced.Compared with normal group,replenishing spleen group increased expression of AQP1、2、3 and cAMP/PKA pathway in the bama mini-pigs kidney.Conclusion:Spleen deficienly and phlegm atherosclerosis model show morphology of the kidney and decrease of AQP1,2,3,methods for replenishing spleen can regulate abnormal water metabolism regulatory proteins,possibly related to cAMP/PKA pathway.