Effects of Danshen Tongluo Jiedu Decoction on NF-kappa B Mediated Inflammatory Response in Bone Marrow Stem Cell Transplantation Rats With Acute Myocardial Infarction
LI Xin-hui
LI Ya-jing
HUANG Miao-xin
XU Fu-li
XIAO Qing
GUO Chen-he
Abstract：Objective:To explore the effect of Danshen Tongluo Jiedu Decoction on the inflammatory reaction mediated by NF-κB in acute myocardial infarction (AMI) rats by Bone marrow stem cell transplantation.Methods:The whole bone marrow adherent method was adopted for BMSCs separation and culture.The AMI model was established by closing the left anterior descending coronary artery of SD rats.SD rats were randomly divided into SH (sham group),AMI group (acute myocardial infarction group),BMSCs (BMSCs transplantation group),DSTLJD group (Danshen Tongluo Jiedu group),DSTLJD + BMSCs group (Danshen Tongluo Jiedu combined with BMSCs transplantation group),10 in each group.BMSCs cell suspension injected directly into the edge of the myocardial tissue infarction area;Chinese medicine or physiological saline were administered by gavage.4 weeks later,by using enzyme-linked immunosorbent (ELISA) measure each group rats serum TNF α、IL-6 content was detected.Using immunohistochemical method to observe the myocardial NF-κB protein expression,and HE staining to observe the myocardial tissue pathological changes.Results:Compared with The AMI group,BMSCs,DSTLJD group,DSTLJD + BMSCs group rats serum level of TNF-α、IL-6 and myocardial nuclear factor NF-κB protein expression were significantly reduced,the difference has statistically significant,the degree of myocardial injury was significantly improved;DSTLJD + BMSCs group was better than that of pure stem cell transplantation,and simple DSTLJD group,the difference has statistically significant.Conclusion:Bone marrow stem cell transplantation combined with Danshen Tongluo Jiedu Decoction can improve the myocardial injury,reduced the inflammatory reaction,and repair the ischemic myocardium in AMI rats.The mechanisms may be related to regulate the inflammatory reaction mediated by NF-κB.
Keywords：Danshen Tongluo Jiedu DecoctionAcute myocardial infarctionNF-κBIL-6TNF-αInflammatory reaction
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 931-933,962 )