Effect of Total Saponins of Mao Dong Qing On Cerebral Ischemia Reperfusion Model in Mice
CAO Li-hua
LIU Rui-li
MIAO Ming-San
Abstract：Objective:To explore the action characters of total Saponin from Radix Ilex Pubescens on Cerebral Ischemia-reperfusion injury and its mechanism.Methods:The mice were randomly divided into 7 groups,The sham operation group,Model group,Nimodipine group,Ginaton group,Large,medium and small dose of total Saponin from Radix Ilex Pubescens.Firstly by using the method of blocking the bilateral common carotid artery(CCA) 10 minutes,restore perfusion 10 minutes,and then Bilateral carotid artery occlusion 10 minutes.After the last administration and then reperfusion 24 hours.To discuss the protection of saponin from Radix Ilex Pubescens for Cerebral Ischemia-reperfusion injury.The activity of nitricoxide synthase (NOS) and the level of nitrogen monoxidum (NO),the content of SOD and MDA.The morphological changes of brain tissue were observed.Results:The models with cerebral ischemia-reperfusion injury in mice were copied successfully,The activity of NOS and the content of NO were increased significantly and the ATP were lowerd significantly of the animals brain in surgical model,Reduce nerve cell injury histopathology.The better group is high dose and medium dose.Conclusion:The Saponin of Radix Ilex Pubescentis show protective effect on improving cerebral ischemic conditions of Cerebral Ischemia-reperfusion mice.
Keywords：Saponin of Radix Ilex PubescentisCerebral Ischemia reperfusionnerve cell
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 928-930,943 )