Effects of Ning Shen Granule on the Apoptosis of Renal Mesangial Cells in Iga Nephropathy Rats

ZHAO Lin; WANG Zhi-xue; QI Fang-hua; MU Lin-mao; LI An-yuan

Abstract：Objective:To research the effects of Ningshen Granule on apoptosis of mesangial cells (MsC) in rats with IgA nephropathy.Methods Thirty-two rats were randomly divided into normal control group,model group,Ningshen granula low-dose and high-dose groups (12 and 24 g/kg).The IgA nephropathy rats were established by lipopolysaccharide,bovine serum albumin and carbon tetrachloride.After the models were established,the rats of the four groups were administrated in the morning and evening on every day for consecutive 4 weeks.After the above treatment,red blood cell count of urine,24 hours urine protein quantity,MsC apoptosis rate were detected.Expression of Bax protein of renal cortex tissue was detected by Western blot.Results:Compared with the control group,the red blood cell count of urine,24 hours urine protein quantity,MsC apoptosis rate and the expression of Bax protein were significantly increased.Compared with the model group,the red blood cell count of urine and 24 hours urine protein quantity of the rats in Ningshen granula lowdose and high-dose groups were decreased.Compared with the model group,the MsC apoptosis rate and the expression of Bax protein in Ningshen granula high-dose group were decreased significantly.Conclusion:Ningshen granula could relieve the hematuria and albuminuria,and its mechanism may be related to up-regulation the expression of Bax protein in MsC,enhancement the apoptosis and inhibited the proliferation of MsC.