Study on Evaluation of Interstitial Cells of Cajal Model in Dampness Obstructing Spleen and Stomach Syndrome Based on the Na-K-ATP Enzyme Activity Changes
WANG Qi-yue
YANG Xu
WANG Ji-e
CHEN Ji-lan
HUANG Xiu-shen
Abstract：Objective:Establishement and evaluature of of interstitial cells of Cajal spleen and stromach bloked by dampness syndrone in vitro study.Methods:The dampness obstructing spleen stomach syndrome animal model establishment and animal serum preparation;ICC cultured in vitro by collagenase Ⅱ,The dampness containing animal serum in vitro model of middle energizer dampness obstructing spleen stomach syndrome of interstitial cells of Cajal model,Simulation of rat model in vivo ICC growth,Pingwei power and serum regulating mechanism of ICC cells in the treatment of dampness syndrome.The successful establishment of cell model counterevidence syndrome.Results:After the cell model was made,the Na +-K +-ATP enzyme activity decreased,K + concentration and ATP increased,and the anaerobic metabolism ability of ICC cells was enhanced.Wet resistance of middle energizer cells through a flat stomach scattered serum intervention,and natural recovery group compared,fiat stomach scattered group ICC Na +-K +-ATPase and K activity was significantly increased,the decline in ATP and LDH activity decreased.Conclusion:The dampness obstructing spleen stomach syndrome ICC model cells and normal ICC have differences.The successful cell model was established successfully,the sodium pump activity is the basic pathological change in the change of the dampness obstructing spleen stomach syndrome,and the level of the stomach may be through the recovery of sodium potassium pump activity,Blocking the downward trend of K+ concentration of dampness obstructing spleen stomach syndrome in the Cajal interstitial cell metabolism.
Keywords：TCM Syndrome ModelThe dampness obstructing spleen stomach syndrome rat modelPing Wei powder
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 630-633 )