Protective Effect of Angelic Decoction on Acute Liver Injury Mice Induced by Concanavalin A
CHEN Xin
MA Ya-luan
LI Rui
ZHU Liu-luan
WANG Xian-bo
ZHANG Jian-ping
Abstract：Objective:To investigate the protective effect of Angelic decoction on acute liver injury mice induced by Concanavalin A (ConA).Methods:Preparing acute liver injury mice model,6-8 weeks old C57BL/6 male mice were adopted by intravenous injection of ConA.The survival rate was observed on the lethal model.For the sub-lethal model,10hour later,the mice were sacrificed to measure the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST)and liver HE staining;at 3h and 10h,the serum cytokines were measured by Cytometric Bead Array;the liver inducible nitric oxide synthase was measured at 3h and the spleen interferon-γ was measured at 10h by realtime PCR.Results:Compared with normal mice,after giving treatment of Angelic decoction,the survival rate of acute liver injury model was increased remarkable,the liver tissue injury were reduced and the serum levels of ALT,AST were decreased,the serum inflammatory cytokines IL-12,IL-6 and IFN-γwere decreased,the liver iNOS mRNA expression were decreased,but had no effect on the spleen IFN-γ mRNA expression.Conclusion:Angelic decoction has anti-inflammatory and liver protection effect on acute liver injury induced by ConA in mice.
Keywords：Angelic decoctionConcanavalin AAcute liver injuryCytokine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 492-495,531 )