Analgesia effect of Contrary Medicine Combination of processed Aconiti Radix Cocta and Rhizoma pinelliae, Fructus trichosanthis, Bulbus fritillariae thunbergli,Bletilla striata in Formalin-induced Pain Mice

WANG Qian-qian; SUN Dan-ni; LIU Chun-fang; WANG Chao; WANG Hui; Sun Cong-cong; HE Lian-hua; LIN Na

Abstract：Objective:To investigate the analgesic effect and mechanism of the combined administration of Aconiti Radix Cocta with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae thunbergli or Bletilla striata in formalininduced pain mice.Methods:Formalin was subcutaneously injected into the plantar surface of ICR mice to induce a model with two phases of nociceptive licking responsiveness.The analgesic effects of the different combined administration of traditional Chinese medicine (TCM) and Aconiti Radix Cocta were observed.Then the analgesic effects of the combination of antagonist Nor-BNI or κ-opioid receptor agonist U-69593 with Aconiti Radix Cocta in Formalin-induced pain mice were investigated,and the levels of NO,PGE2 and TNF-α in injured skin tissue were detected by ELISA.Results:Compared with Aconiti Radix Cocta-treated group,the combined administration of Aconiti Radix Cocta with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae thunbergli or Bletilla striata increased the licking time of two phases in formalin-induced pain mice,with peak at the rate of 1 ∶ 3.Nor-BNI could reduce the analgesic effect of Aconiti Radix Cocta.The combination of Aconiti Radix Cocta and Rhizoma pinelliae or Bletilla striata could partly reduce the analgesic effect of U-69593.Furthermore,Aconiti Radix Cocta could significantly reduce levels of NO,PGE2 and TNF-α in the skin of inflamed paw,while this effects were significantly decreased when the co-administration with Aconiti Radix Cocta and Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae thunbergli or Bletilla striata.Conclusion:The analgesic effect of Aconiti Radix Cocta was decreased markedly when the co-administration with Aconiti Radix Cocta and Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae thunbergli or Bletilla striata.It may be related to the antagonistic action of κ opioid receptor and the decreased anti-inflammatory effect of Aconiti Radix Cocta on NO,PGE2 and TNF-α.This study provides the experimental basis for further study on compatibility of Aconiti Radix Cocta with Rhizoma pinelliae,Fructus trichosanthis,Bulbus fritillariae thunbergli or Bletilla striata.