Effects of New Xuefu Zhuyu Decoction on the Expression of Immunomodulatory Factors HMGB-1 and Foxp3 in Atherosclerosis Model Rats

JIANG Ting; JI Wen-yan; SUI Yong-ni; LIU Ni-na; JI Zhong-qiang

Abstract：Objective:By observing the effect of New Xuefu Zhuyu Decoction on aorta pathologymorphology and HMGB-1,Foxp3 expression in atherosclerosis model rats,explore its anti atherosclerosis mechanism by the regulation of immune factors.Methods:48 healthy male Wistar rats were randomly divided into blank control group,model group,western medicine group,low dose Chinese medicine group,high dose Chinese medicine group,and the group of high dose Chinese medicine plus western medicine.Except the blank group,other groups were copied to atherosclerosis model,low dose group and high dose group of Chinese medicine garaged with New Xuefu Zhuyu Decoction at a concentration of 9 g/ (kg · d) and 18g/ (kg · d) respectively;western medicine group with aqueous solution of atorvastatin calcium 2 mg/(kg · d);these two programs administered by adding for Integrative Medicine group;simultaneously,the control and the model group were fed with volume of normal saline.After 8 weeks,the thoracic aortic pathology change was observed under light microscope with HE staining;the HMGB-1,Foxp3 expression of thoracic aortic was detected by immunohistochemical method,and then their correlativity was analyzed.Results:Compared with the model group,the New Xuefu Zhuyu Decoction could decrease the expression of HMGB-1 and increase the expression of Foxp3,and the two have a significant negative correlation.Conclusion:New Xuefu Zhuyu Decoction could down regulate proinflammatory factor HMGB-1 and upregulation of anti-inflammatory factor Foxp3 expression,to play the role of anti atherosclerosis by regulating the balance of inflammatory factor.