Study on Metabolism of Acute Blood Stasis Rat Model Based on the Liquid Chromatography-Mass Spectrometry
HUANG Shuo
SUN Ming-qian
SUN Lei
LIU Jian-xun
Abstract：Objective:A metabonomics approach based on liquid chromatography coupled time-of-flight mass spectrometry (LC-TOF-MS) was utilized to obtain potential biomarkers of acute blood stasis rat and investigate metabolic mechanism of this TCM syndrome animal model.Method:SD rats were divided into normal group,blood stasis model group.Reversed-phase chromatography was used to separate the endogenous metabolites in serum.Partial least-squares to latent structure-discriminat analysis (PLS-DA) were used for data analysis.Result:Good separations were observed between the normal and model group.The major disturbed metabolic pathways were fatty acids,phosphatidylcholines and sphingolipids metabolism.Conclusion:The study provided further understanding of pathologic mechanism of the TCM syndrome animal model.
Keywords：Blood stasisMetabonomicsBiomarkersLC-MS
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 332-334 )