The Effects of PNS on the PLA2G4A And it's Downstream Products in Mimic Ischemic Brain Microvascular Endothelial Cells
SU Jing
LI Xing-guang
LI Wei-hong
HU Yan-hong
MA Jia-bao
ZHANG Sai
HU Jing-hong
WANG Shu-yan
KANG So-yeon
Abstract：Objective to evaluate the effects of Panax Notoginseng Saponins (PNS) on the PLA2G4A in Mimic ischemia brain microvascular endothelial cells (BMECs).Methods:primary rat BMECs were used and mimic cerebral ischemia in vitro was established by oxygen-glucose-deprived(OGD) method.OGD-induced BMECs were treated with PNS and Pyrrophenone(PLA2G4A inhibitor)respectively.The expression of PLA2G4A in mRNA and protein were detected by RT-PCR and Western blot methods.The expression of AA 20-HETE TXA2/PGI2 were detected by Elisa method.Results:both mRNA and protein expression of PLA2G4A in OGD-induced BMECs were down-regulated by PNS.AA 20-HETE TXA2/PGI2 were also decreased in administration group.Conclusion:PNS can down regulate the expression and function of PLA2G4A,which may be the internal mechanism of PNS's Anticoagulant effect in cerebral ischemia.
Keywords：Panax Notoginseng Saponinsbrain microvascular endothelial cellsPLA2G4AAA20-HETE
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 196-198 )