Establishment and Evaluation of Spleen Deficiency Syndrome Based on the Obesity Mice Model
SI Yuan-cheng
MIAO Wei-na
HE Jia-yue
DING Wei-jun
Abstract：Objective:To copy and evaluate the spleen deficiency syndrome type in obese mice.Method:Replicating obese mice with high-fat diet,and on this basis,according to the spleen deficient fatigue,less food,stay,loose stools and secondary screening eligible obese mice,and through the ELISA method to detect serum total cholesterol,triglycerides,high-density lipoprotein and low density lipoprotein content,observe serum inflammatory factors (interleukin 6,interleukin 10 and tumor necrosis factor alpha) and CD4 +,CD8 +,CD11c + content changes of the spleen,thymus and intestinal corpuscle.Results:The model of obesity mice was 62.96％,23.53％ of which was spleen deficiency syndrome type.The IL-1O,the TNF alpha of these mice is significantly higher than normal group,interleukin-6 was significantly lower than normal group.The CD4 +,CD8 + and CD11c + of their gut peyer's patch were also significantly reduced.Conclusion:This method could better screen spleen deficiency syndrome by obesity animal medel.
Keywords：Spleen deficiency syndrome typeObesityAnimal modelsLack of powerWater contentImmune
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 177-179,186 )