Experimental study on Chai Hu Long Mu Decoction on Lewis lung cancer in mice

HUANG Yan; LIU Xin; LIAO Duan-Fang

Abstract：Objective:To observe and evaluate Chai Hu Long Mu Decoction radiation therapy and cisplatin efficacy of Lewis lung carcinoma in mice.Methods:The 40 C57BL/6J mice inoculated with Lewis lung routine,successful modeling were randomly divided into four groups,namely Chai Hu Long Mu Decoction group,model group,cisplatin control group and the control group endostatin combined with NP.Treatment time is 2 weeks.The tumor weight inhibition rate was calculated,the number of pulmonary nodules statistical computing anti lung metastasis,tumor cells using MTT assay valueadded ability to detect blood red ceils,platelets,white blood cells and the ratio of spleen natural killer cell activity,IFN-γ and IL-10 levels and the ratio of T lymphocytes.Results:Chai Hu Long Mu Decoction; Experimental Research,Group lung tumor weight and the number of metastatic nodules was significantly reduced (P ＜ 0.05) ; Chai Hu Long Mu Decoction group at two and three days the number of tumor cells was significantly lower than the model group (P ＜ 0.05) ; model group,cisplatin control group and the treatment group endostatin combined with NP significantly reduced natural killer cell activity,IFN-γ were significantly reduced,IL-10 was significantly higher (P ＜ 0.05).Chai Hu Long Mu Decoction group Longmu number of leukocytes was significantly higher than the other groups (P ＜ 0.05),splenic T lymphocytes CD4 +,CD4 +/CD8 + ratio was significantly higher than the model group,the cisplatin control group and the treatment group endostatin combined with NP,difference was statistically significant (P ＜ 0.05) Conclusion:Bupleurum Long Mu Tang on Lewis lung tumor cells significantly inhibited.