Study on the effect of bone, skeletal muscle isocitrate dehydrogenase of osteoporosis mice by the Bushen Jianpi herbs
LI Jia
YANG Fang
ZHENG Hong-xin
WANG Yong-jun
Abstract：Objective:To compare the effect of invigorating kidney,invigorating spleen in treating osteoporosis by investigating expression of IDH in the skeleton and skeletal muscle of osteoporosis mice.Method:Except the normal control group,OPG knockout mice were taken as the osteoporosis model.OPG-/-mice were randomly divided into model control group (no herb inducted),invigorating kidney group,invigorating spleen group and Fosamax group.Euzymelinked immunosorbent assay were taken to analyze the content of IDH in skeletal and muscle.Result:All the OPG-/-mice shows significantly lower IDH in the muscle than normal control group.Invigorating kidney dramatically reverse the decrease of IDH expression(P ＜ 0.0l),and also Fosamax (P ＜ 0.01).However,the invigorating spleen group doesn ' t show statistically significant change.Similar result turns out in skeletal.Conclusion:①Osteoporosis causes the failure of bone and muscle in function,which is marked with the lower IDH expression in parallel to the energetic metabolism.② Treating with invigorating the kidney and invigorating the spleen can partially reverse IDH expression in the skeleton and skeletal muscle,which indicates that it can efficiently prevent and cure osteoporosis.
Keywords：osteoporosisIDHskeletal muscle
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)