The Experimental Study of the Effect of Shenmai Injecton on the Expression of Heat Shock Proteins 70 in the Rat Brain after Intracerebral Hemorrhage

HUANG Ren-fa; KANG Lei; HE Ze-yun; TENG Jiu-xiang

Abstract：Objective:To observe the effects of ShenMai Injection on the expression of the heat shock proteins70 (HSP70) in the areas of hematoma,ischamic Penumbra(IP),hippocampal gyrus and cotex of operated sides.Methods:The 50 rats were randomized into 4 groups:normal groups,false operation groups,modeling groups,and treating groups with Shenmai Injection(SM groups),the rats in the modeling groups and treating groups with Shenmai were made into four stages respectively in 1d,3d,Sd,7d after operation,the models of Intracerebral Hemorrhage were made by injecting Collagenase type Ⅶ stereotaxically;we evaluated the pathosis of all the rats,and counted the scores of climbing haulm,the pathological changes of neurocyte were observed by electron microscope.Subsquently,we observed the expression of heat shock proteins 70 in the perihematomal area by immunohistochem at the different stages in the the areas of hematoma,IP,hippocampal gyrus and cotex of operated sides,and the effect of Shenmai Injecton on them.Result:Rats in the normal groups and false groups indicated no pathological signs.However,Rats in the modeling groups and SM groups desmonstrated obvious pathological signs.The observation by the electron microscope displayed that the rat,s cytoarchitecture in the normal groups and false groups is normal,that the cytoarchitecture in the modeling groups is not integrated,the apoptosis bodies found in their cytoarchitecture were more than them in the SM groups.A few of expression of HSP70 can be observed in the rats of normal groups and false operation groups.However,the expression of HSP70 increased significantly in the areas of IP,hippocampal gyrus and cotex of operated sides,got to a peak at 3st day,followly decreased gradually,kept higher at 7st day.SM Injection can enhance the levels of the expression of HSP70.there were significant difference between modeling groups and SM groups.Conclusion:SM Injection can promote expression of HSP70,which can inhibt neuronal apoptosis,and increase the ability of neuronal tolerance to hypoxia,provide a protctive contribution.